FastQCFastQC Report
Mon 29 Jun 2015
G85_M3_AGTCAA_L008_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M3_AGTCAA_L008_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20249167
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA57150.034.53911246-47
GATCGGA140350.031.8070581
GAGCGTC106900.030.0150669
AAGAGCG124150.029.965087
AGAGCGT108650.029.6603138
ATCGGAA155200.028.5845972
CCGTATC74300.026.84838548-49
GATCTCG89500.026.02947834-35
GTCGCCG76050.025.92485444-45
TCGTGTA72050.025.71865814-15
CGGAAGA170500.025.0654284
CGTATCA60950.024.26068948-49
GCCGTAT67600.022.52756746-47
TCGCCGT59950.021.87306244-45
TCGGAAG195050.021.7194023
CGTCGTG95350.020.531412-13
AGCGTCG106800.019.8544210-11
TCTCGGT92100.019.74094236-37
TAGATCT113000.018.9908132-33
GTCGTGT74450.018.80004912-13
Produced by FastQC (version 0.11.2)