FastQCFastQC Report
Tue 12 Apr 2016
Waxman-TP19_GCCAAT_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP19_GCCAAT_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGC550.002858399233.8196379
GTATTGC650.00647962328.6108916
TCGGTTT700.00929340226.5725732
CGGTTTG700.00930247226.5672573
AGAATCG700.00930247226.5672578
GCGCGAT450.001012007125.83444434-35
CGCTGCA950.001386703124.4698414
TCGCAAT500.001869638623.25152-53
AGTCAAG1303.3898005E-421.4581686
CATAACA1150.00420629420.2142187
GTGGAAT1657.357566E-519.7478731
AGATTGG1250.006811032518.597084
TCCGGCC650.00854131117.88538454-55
GAATCTG1300.00853953517.8818074
CCAACTA1300.00853953517.8818078
CCGGGCT1650.00167119416.9267481
CTGAGAC2551.2190685E-414.603471
AAGACGT1159.364425E-414.15278214-15
ACAATCC1750.001776510410.62902836-37
CTTTTCT4200.0079259068.8663921
Produced by FastQC (version 0.11.2)