FastQCFastQC Report
Fri 27 May 2016
Waxman-TP19_GCCAAT_L007_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP19_GCCAAT_L007_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29319981
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA48900.017.8774146-47
CGATCAC60450.012.8448963
GATCTCG91450.011.84659134-35
CGTATCA52450.011.43658950-51
CGCGTAT6252.518027E-811.1589071
GTCGCCG84000.010.93305744-45
ACGATCA66150.010.8243372
CCGTATC85350.010.81459848-49
TCGTGTA86400.010.46680414-15
CTCGTTT74650.010.3392421
CACGATC81650.010.1931231
TCGCCGT60450.09.96159946-47
CGCGGAT29450.09.9463941
CGATTAG46350.09.9310664
ATTGGAC113750.09.5647786
CCGGAAT90000.09.4540751
CGCGTAA11405.456968E-129.380661
CGGCGAT23900.09.3379971
GCCGTAT66000.09.15910148-49
CTAGCCT145050.09.07151
Produced by FastQC (version 0.11.2)