FastQCFastQC Report
Fri 27 May 2016
Waxman-TP19_GCCAAT_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP19_GCCAAT_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29319981
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT89900.019.01052542-43
TATGCCG91450.018.50824746-47
CGTATGC100050.017.24453544-45
ATCTCGT106100.015.95447440-41
CGATCAC60550.015.0506063
AGTCACG120050.014.19549528-29
TCACGCC120400.014.13755930-31
ACGCCAA122650.014.06808932-33
ACGATCA68050.013.1193792
TATCTCG83700.013.05674338-39
CACGATC83900.011.9238571
ATATCTC152800.011.3217138-39
CCGTCTT156050.011.29333850-51
TGCCGTC152300.011.23550448-49
CGCCAAT98600.011.10817532-33
CCGGAAT86050.010.9770441
GATCGGA195450.010.9750251
GTCACGC102200.010.78291428-29
CGTCTGA166650.010.21325416-17
ATCGGAA216850.09.9065572
Produced by FastQC (version 0.11.2)