FastQCFastQC Report
Fri 27 May 2016
Waxman-TP18_ACAGTG_L007_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP18_ACAGTG_L007_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39785793
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT431230.10838793636713487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC124150.025.5047383
ACGATCA129800.024.8960572
CACGATC153400.022.1873861
CGCCGTA66550.016.24539446-47
CCACGAT145200.014.0578321
ATTGGAC355650.013.0998216
TCACAAG268800.012.7138926
AAGACGT247400.012.197353
CGTATCA66400.011.76501548-49
AATTGGA436950.011.535015
AAAGACG272050.011.5194462
GAATTGG444800.011.4986894
AGACGTG270700.011.3879554
GGAATTG502600.011.3604713
GATCTCG127500.011.10465134-35
ACGTGGT280250.011.0164816
CGCCATT86900.010.9686841
GACGTGG285350.010.8195855
CCGTATC105950.010.70878348-49
CTGGAAT680050.010.6865751
Produced by FastQC (version 0.11.2)