FastQCFastQC Report
Tue 12 Jul 2016
SRR769953_IMR90_DNASE_GENOMIC.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR769953_IMR90_DNASE_GENOMIC.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115350357
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT1720260.14913347862460452Illumina Paired End PCR Primer 2 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG323800.018.2404520
ATGCCGA375450.018.19103414
CGGTTCA381200.018.1553571
AATGCCG388700.017.73228513
ATCGGAA344200.017.05773222
TGCCGAG403000.016.99884815
TCGGAAG349750.016.92856223
AGCGGTT335850.016.72295830
CTCGTAT49600.016.6818728
GATCGGA357250.016.52883521
GAGCGGT344800.016.47714629
GCGGTTC143750.016.4527811
CGAGATC392150.015.93058118
CGGAAGA376500.015.79163724
TCTCGTA53350.015.78514227
AAGAGCG374100.015.68535827
AGAGCGG380850.015.51210228
TCGTATG54950.015.19646529
CGTATGC57200.014.65466930
GAGATCG430850.014.46317819
Produced by FastQC (version 0.11.2)