FastQCFastQC Report
Tue 12 Jul 2016
SRR352450_GM12878_DNASE_CHROMATIN_DUKE.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR352450_GM12878_DNASE_CHROMATIN_DUKE.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87464102
Sequences flagged as poor quality0
Sequence length20
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTATGCCGTCTTCTGCTT8939481.0220741762146028No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT970000.013.2296427
TGCCGTC971950.013.196686
CCGTCTT977000.013.1707728
TCGTATG972700.013.1610881
TATGCCG973700.013.157484
ATGCCGT974150.013.1538535
CGTCTTC980400.013.1114569
CGTATGC983350.013.0258542
GTATGCC1001500.012.8036973
GTCTTCT1067800.012.13821610
TTCTGCT1088250.011.89757613
CTTCTGC1096700.011.80925512
TCTGCTT1094950.011.79708614
TCTTCTG1136050.011.41289211
GTCGGAC6250.07.7420851
Produced by FastQC (version 0.11.2)