FastQCFastQC Report
Tue 12 Jul 2016
SRR352448_GM12878_DNASE_CHROMATIN_DUKE.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR352448_GM12878_DNASE_CHROMATIN_DUKE.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144500455
Sequences flagged as poor quality0
Sequence length20
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAGAGTTCTACAGTCCG102160437.069903689922636No Hit
GGTTCAGAGTTCTACAGTCC9393180.6500450119689934No Hit
AAAAAAAAAAAAAAAAAAAA4753040.3289290680780209No Hit
TCGTATGCCGTCTTCTGCTT2797090.19356963270461675No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCCG10621500.013.818356514
GTCGGAC204500.013.6177021
TCGGACT214100.012.9620582
GGTTCAG1165450.012.74811
GTTCAGA12133800.012.6711861
GTTCTAC11908900.012.6633418
AGTTCTA12015400.012.6097347
AGAGTTC12092550.012.6060715
TACAGTC11768000.012.59890812
TCAGAGT12173800.012.5965543
CTACAGT11821200.012.594892511
GAGTTCT12124900.012.58958156
TCTACAG11878400.012.58593610
ACAGTCC11673100.012.57451913
TTCTACA11917700.012.5738079
CGGACTG216300.012.5224823
TTCAGAG12252150.012.515232
CAGAGTT12253400.012.4985824
GTATCGT71700.012.1630219
TGTATCG72750.012.1209988
Produced by FastQC (version 0.11.2)