FastQCFastQC Report
Tue 12 Jul 2016
SRR231245_GM12878_DNASE_CHROMATIN_UW.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR231245_GM12878_DNASE_CHROMATIN_UW.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175128655
Sequences flagged as poor quality0
Sequence length28-36
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT415650.024.53164712
TCGTATG432750.023.86788213
GCTCGTA453100.023.81363511
CGTATGC463050.022.515414
AGCTCGT488000.022.27415710
ATCGGAA690650.021.7365632
GATCGGA701800.021.6156481
TCGGAAG713850.021.3177173
TATGCCG488950.020.69534116
ATGCCGT495650.019.9277417
GAGCTCG655000.019.6588069
CCGTCTT501450.019.30080220
GTATGCC536400.019.18834115
CGTCTTC501800.018.84641621
TGCCGTC522000.018.54724918
GCCGTCT558550.017.53999119
CGGAAGA904750.017.0717224
AGAGCTC799300.016.338588
GTCTTCT579800.016.03788222
AAGAGCT899850.015.0827377
Produced by FastQC (version 0.11.2)