FastQCFastQC Report
Tue 12 Jul 2016
SRR231117_GM12878_DNASE_CHROMATIN_UW.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR231117_GM12878_DNASE_CHROMATIN_UW.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36664098
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT8329702.271895520244355Illumina Paired End PCR Primer 2 (96% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCG3807851.0385773025153926Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG783120.21359314498886622Illumina Paired End PCR Primer 2 (100% over 36bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAA399230.1088885372278898Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGC397510.1084194134545462Illumina Paired End PCR Primer 2 (100% over 36bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC387990.10582286791836527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT52550.024.8913628
CGTATGC55350.024.01273730
TCTCGTA54550.023.92326527
TCGTATG55050.023.89775829
GCGGTTC715000.023.68418111
ACCGATC57500.023.34769622
CCGATCT57300.022.87967523
GACCGAT60900.022.7084921
ATCTCGT58700.022.12937726
AGACCGA74350.019.26605820
CCGAGAT1646350.018.35181417
TGCCGAG1747150.018.15688715
ATGCCGA1754200.018.06781814
CGAGATC1666100.018.04589718
CGGTTCA1743950.017.992911
AATGCCG1769550.017.96984713
GAATGCC1799650.017.72253812
GTTCAGC1772600.017.6815093
GCCGAGA1826100.017.49094416
TCAGCAG1823500.017.4851635
Produced by FastQC (version 0.11.2)