FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M30_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M30_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27619991
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGCTTTAAACTCTTTGGGGACATATGTTTCATCAACTGTCAGAGCAG379840.13752357848342528No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282030.10211082255602473No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT142400.016.630635
ATCATGC149500.016.1778537
TATCATG143300.016.1243296
TAAACTC408950.016.0526759
CTCGAAT120450.015.5030071
CTCCGTT171200.015.0402041
AGCTTTA491700.014.83634954
CGAATGT99300.014.72456553
AGTATCA188700.013.1663384
TTTAAAC529300.012.9874627
TTAAACT532400.012.6684748
CGTTCTA39250.012.47633654
TCGGTTT141150.012.3999832
GCTTTAA597600.012.394465
CTCCTAT125500.012.3799441
CTCCCAT302350.012.2074031
GTCAGTA246300.011.9942591
TCCGATT136400.011.5644632
CAGTATC222650.011.4842163
CTCGGTT115250.011.4556681
Produced by FastQC (version 0.11.2)