FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M29_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M29_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27261787
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGCTTTAAACTCTTTGGGGACATATGTTTCATCAACTGTCAGAGCAG405590.14877601383944494No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364760.13379900591256177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACTC448950.014.7986189
TATCATG124700.014.7771596
GTATCAT128450.014.4578265
ATCATGC138200.014.0107587
CTCCGTT165500.013.40131
CTCGAAT116650.013.3844451
CGAATGT93500.013.1723023
AGCTTTA536050.013.125034
TCCGATT124800.012.2348442
TTTAAAC568050.012.1520487
TCGGTTT129300.012.1432552
TTCGATT78350.011.9503742
TTAAACT585700.011.8227168
CTCCCAT276400.011.7196941
GCTTTAA676100.011.3704525
TCGATAC32000.011.0286732
CGGGCTA35800.011.0047981
CTCGATT39300.010.76731
AGTATCA177100.010.734864
GCGGAAT61850.010.6163241
Produced by FastQC (version 0.11.2)