FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M28_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M28_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27252891
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT325210.11933045928962178No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT273430.10033064015116781No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC112500.037.240993
CACGATC130500.032.683651
ACGATCA158750.026.672432
GCCACGA75750.018.6073071
CCACGAT119650.018.2838841
CCACTAG118450.017.9890163
TCACAAG254650.016.9040496
ACGTGGT310600.014.253026
GATCACA316700.014.1153594
GTTTCGG80000.013.2274384
CACTAGC165000.013.2192574
GACGTGG373700.011.9040695
CGTGGTC330000.011.7573127
AAGACGT382050.011.5313993
CTTAGAT142050.011.4729911
GAAAGAC477550.011.31421
ACTAGCC197550.011.1135515
AGACGTG404000.010.8157264
TAATGTA552000.010.7848674
AATGTAG549750.010.6321445
Produced by FastQC (version 0.11.2)