FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M28_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M28_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27252891
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC341670.12537018549701753No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT306930.11262291402405712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAC42400.017.8361022
TATCATG136050.015.7666416
ATCATGC143300.015.5717337
GTATCAT137750.015.5198085
CTCGAAT121800.015.0323281
CGAATGT100050.013.8216743
AGTATCA173600.013.1913044
ATTGGAC368400.013.16927056
CTCGATA54850.012.9001531
TTCGATT74950.012.7807552
TCCGATT130400.012.5378992
CCGATTA116800.011.8395413
CAGTATC201900.011.6650863
CGGGATA35050.011.6546651
CTTCGAT81100.011.6029571
AATTGGA486050.011.5662225
CTCCTAT117300.011.4424661
TTACGAC21000.011.3113945
CTCCGTT131300.011.2779741
TCGGTTT119500.011.2706812
Produced by FastQC (version 0.11.2)