FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M27_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M27_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26812804
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT275790.10285757506003476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC98500.036.1032073
CACGATC133950.026.9109571
ACGATCA156200.022.9120142
GCCACGA74650.016.718971
TCACAAG254750.014.9479336
CCACTAG119200.014.9168033
ACGTGGT293900.014.5487796
GTTTCGG73700.013.1862264
GACGTGG345750.012.7623735
GATCACA314200.012.6240774
CGTGGTC304600.012.5015957
CCACGAT118000.012.1945131
AAGACGT363850.011.9698323
CACTAGC156900.011.9290174
AGACGTG376450.011.68390754
GAAAGAC462750.011.2166561
AAAGACG409650.010.7051372
CTTAGAT156750.010.4445011
ACTAGCC182550.010.3312445
TAATGTA506200.09.9263034
Produced by FastQC (version 0.11.2)