FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M27_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M27_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26812804
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291430.10869060915822157No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC279550.10425989016292365No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAC36500.014.4056392
CTCGAAT133850.014.005621
ATCATGC145550.013.5014097
GTATCAT141850.013.3968685
TATCATG141750.013.3555366
TCCGATT135850.012.9369752
CGAATGT99700.012.7151073
ATTGGAC307450.012.6663496
CTCGATA44650.012.4159431
CCGATTA128250.012.2433893
CTCCGTT131250.011.89329051
TCGGTTT125000.011.524512
CTCCTAT126900.011.4962441
TTACGAC21350.011.4633025
AATTGGA430500.011.3032215
AGTATCA179850.010.9715324
CTCCGAT231250.010.9139491
CGATTAG133550.010.8387534
GTCAGTA235450.010.6573031
CTTAGGT415500.010.6562711
Produced by FastQC (version 0.11.2)