FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M26_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M26_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21987787
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG120250.025.6812213
CACTAGC174750.018.0426544
CCCACTA188150.016.878622
ACTAGCC191900.016.6922045
TAGCCTC201050.015.7536167
CCCCACT241400.014.6587381
CTAGCCT270200.011.6420036
GTTTCGG67400.011.4279254
CGGAATT92150.011.3311812
CGACAAT40150.010.7883061
CGCCGTA232250.010.105613140-144
CGACGAT24000.09.7887121
CTTAGAT114750.09.6607551
GCCGTAT226800.09.573904140-144
TCGCCGT268200.09.55098140-144
TAGATCC111850.09.3963753
CCGTATC208450.09.366742140-144
CGTATCA188450.09.35989140-144
TTTCGGA92100.09.3007085
TCTCGGT580900.09.19857140-144
Produced by FastQC (version 0.11.2)