FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M26_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M26_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21987787
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGCTTTAAACTCTTTGGGGACATATGTTTCATCAACTGTCAGAGCAG298340.1356844142614261No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272140.12376870851077465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT88350.016.0152381
CTCGATT37500.014.1980861
TAAACTC350150.013.5677539
CGGGCTA26750.013.0874691
CGAATGT72200.013.0683253
GTATCAT103000.012.8587495
TATCATG104100.012.7228746
TCGATAC25300.012.5261612
CTCCTAT103200.012.5092641
ACGTTCG15600.012.4582586
CGTCGCG18350.012.1602859
ATCATGC111550.012.0022187
TCCGATT104250.011.8142432
AGCTTTA423500.011.8007854
CTCGTTT117400.011.6795991
TCGGTTT102400.011.6759972
CTTAGGG213400.011.517911
CTCCGAT191350.011.2442831
TTTAAAC442550.011.109047
GCTTTAA522850.011.0824795
Produced by FastQC (version 0.11.2)