FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M25_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M25_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29026014
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421460.1452007843722531No Hit
CTCAGCTTTAAACTCTTTGGGGACATATGTTTCATCAACTGTCAGAGCAG385080.132667199843561No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT43400.015.2940451
TATCATG143600.013.8849666
ATCATGC155300.013.3023987
TCGATAC36500.013.2209242
TAAACTC461000.013.2095789
GTATCAT147900.013.1892655
CTCCGTT166750.012.641621
TCCGATT138150.012.5123882
CTCGAAT121700.011.986991
CTCGATA48500.011.7306911
AGCTTTA567350.011.613784
CTTAGGG291300.011.2428541
CTCGTTT152700.011.082041
TCGGTTT141600.010.9867782
CTCCGAT250000.010.9411261
CTCCTAT131550.010.8676591
TTTAAAC591400.010.856837
CCGATTA143850.010.8149323
CGATTAG136900.010.7833574
AGTATCA193850.010.7358294
Produced by FastQC (version 0.11.2)