FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M24_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M24_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24017839
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC62900.024.8598061
CGATCAC57650.023.8500633
TATGCCG67200.020.3535333
CCACTAG120750.020.2696973
CACGATC91750.016.482761
TGCCGTC74900.016.0496235
CACTAGC161850.015.3446944
ACTAGCC177050.014.1892575
ATGCCGT91600.014.0672574
CGACGAT20250.013.77613451
ACGATCA104700.013.342722
CCCACTA190050.013.2235142
CTTAGAT129050.012.6857371
TAGATCC117450.012.1357793
GTTTCGG77750.011.7585884
CCGTCTT89900.011.6903587
GTATGCC133650.011.5840072
TAGCCTC216450.011.5067637
GCCGTCT100900.010.9866246
CCCCACT271400.010.6574961
Produced by FastQC (version 0.11.2)