FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M23_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M23_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19127124
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359840.18813074040822866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCTA34350.017.1909074
TCGATAC37650.016.069952
CGGGCTA21200.015.4873881
TCGGTTT98300.015.3874282
TCGGAAT68000.015.2529482
TATCATG87700.014.6918146
GTATCAT87900.014.3308255
CTCGGTT99150.014.2761321
ATCATGC96450.014.0306467
CTCGAAT61100.013.97160151
GTCGATT48100.013.9554641
CGGGGTT87350.013.531741
TCGAAAT32700.013.4363812
GTCCGAT86250.013.3659411
CGGGTAT35100.013.3037611
GTCGGAA114550.013.2485941
GTCGGAT33250.013.1662221
GTCGATA56400.012.9366681
TCCGATA54850.012.8691442
CGAAATA35400.012.6150413
Produced by FastQC (version 0.11.2)