FastQCFastQC Report
Mon 3 Dec 2018
G166_M6.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M6.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4805929
Sequences flagged as poor quality0
Sequence length50
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAG905571.8842766923939158No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA631311.3136065888613835No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC424450.8831799221336811TruSeq Adapter, Index 7 (100% over 50bp)
GGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCG357570.7440184821706688No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT351710.7318252100686464TruSeq Adapter, Index 7 (100% over 50bp)
TCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTC306190.6371088711464526No Hit
GGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGC294500.6127847498371283No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG280110.5828425680029813No Hit
GGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGC273480.5690471082698059No Hit
CGGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAG259770.5405198453826513No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC244220.508163978285988No Hit
GGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGGA210960.4389577956728033No Hit
GGGGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCG210380.4377509530415451No Hit
GGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGC206180.42901174778071005No Hit
CGGGACGCAACGGGTCAGCGAGGCCCTCTCCCTCCCCGAACTCGCAACAC188800.3928480841061115No Hit
CCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGCGC180970.376555708584126No Hit
GGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGG171770.3574126875365824No Hit
GGGTTCCCACGGGGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCG164130.3415156570144919No Hit
CGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGC158250.32928076964932274No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT158130.32903107807044174No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACG156890.326450931755338Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG156820.3263052783343241No Hit
GGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGAC156810.32628447070275074No Hit
GGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTGGTTCGG153510.31941795228352315No Hit
CGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGG144550.3007743143937416No Hit
GACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCAAGAC143710.29902647334157456No Hit
CCAAACACACACAGACAACCACCGCAGGGCGACCGACCGGCCCTCGTCGA142620.2967584415000721No Hit
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA136140.2832750962404979No Hit
GCGAGCGAAGCCGTCGGGGGTGGGACAGGACCAGGCGGGACAGAGAGCGC133000.27674149992644503No Hit
GGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCGGC132600.2759091946635084No Hit
GGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGACCAGGCT132120.27491042834798435No Hit
GGGGGACGGCGGGGGCGAGCCCTGGATAGGCCACCCGTCGGAGCCCGGCG128650.26769018019200863No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC124490.25903420545746725TruSeq Adapter, Index 7 (98% over 50bp)
GGCGACCGACCGGCCCTCGTCGACCGCCACCCCGACAACCCCGCGGCCGC119440.24852635151289165No Hit
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC118160.24586297467149432No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGCCGT114950.23918372493642748TruSeq Adapter, Index 7 (98% over 50bp)
CCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAA114270.23776880598943514No Hit
GCCGACGCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGGAACGGCGG111370.23173459283314424No Hit
GGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCAAGACGAA111000.2309647104649278No Hit
CAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCC109760.22838456414982408No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG109190.22719852915013936No Hit
GGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAG107870.22445192178244833No Hit
GGGACGCCAAACCCTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCC105610.2197493970468561No Hit
ATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCT104470.21737732704748655No Hit
GCATGCGGCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCGAG103370.2150884875744107No Hit
GGCCGCACCCCGTTTCCCAAGACGAACGGCTCTCCGCACCGGACCCCGGT100460.20903346678654636No Hit
CGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCG97320.20249987047249346No Hit
GGGACGCAACGGGTCAGCGAGGCCCTCTCCCTCCCCGAACTCGCAACACC96980.20179241099899728No Hit
TCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACT93710.19498831547448994No Hit
GGACGGACGCGGGACGCCAAACCCTCCCTCGCAACGCCACCGGGGATACC93610.19478023915875578No Hit
GCGGGACGCCAAACCCTCCCTCGCAACGCCACCGGGGATACCACCTCTCT93560.19467620100088867No Hit
GGCGGAGAGCCTCGCGGGAGGACAAACCGGGGGTGAGCCGGGCGGGCGCT91710.19082678915980658No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA90220.18772645205536745No Hit
CCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGACC90050.18737272231861937No Hit
GGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCGCGA88710.18458449968778148No Hit
GCGCCGTGGGAGGGGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGG88640.18443884626676757No Hit
GGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGGA85790.17850867126834374No Hit
GAGGCAGCGGTCCGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGCACG84160.17511702732187678No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC82270.17118438495450097No Hit
GGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGACCAGGCTCACGCGAC80970.1684793928499568No Hit
ACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCG80200.1668772052188037No Hit
GGCCCTCGTCGACCGCCACCCCGACAACCCCGCGGCCGCAGGGGACCGAC78110.16252841021995956No Hit
CCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCG77770.16182095074646338No Hit
GCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGGGGGGAGA77560.1613839904834216No Hit
GGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGG75360.1568063115372699No Hit
GGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGGGGGGAG73860.15368516680125735No Hit
GGGCGCTCGCGCGGAACGGGAGAGTGCATGCGGCCGGACCGACCCCGTCG73560.15306093785405486No Hit
CGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGA72070.14996060074961576No Hit
GGCGGCGGAGAGCCTCGCGGGAGGACAAACCGGGGGTGAGCCGGGCGGGC71750.1492947565392664No Hit
GCGAGGCCAGAAAAGCGTGGCATCGGGACGGGCGAACCCACACGCGACAC71630.1490450649603854No Hit
GGGAACGACACGGGAACCCACGACCGGCACCGACAGGACCGACCCGCGGA70360.14640249575056144No Hit
GGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGC68160.14182481680440973No Hit
GGAAAAACACGGGAACGACACGGGAACCCACGACCGGCACCGACAGGACC65020.1352912204903568No Hit
GCGGACGCGCCCACCCCCGGGCGCCACGGGGGCGCGGTGGGTTCCCACGG64710.1346461839115809No Hit
GGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGT64160.13350176417504295No Hit
CGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAG62990.1310672712809532No Hit
GGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGGGGGGAGACCCCG62970.13102565601780633No Hit
CGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAGGCCAG62000.1290073157551849No Hit
GAAGGAGAAGCGGAGACGAAGAAGAGCCACGGGGGCCACGGGCCCCGACC61290.1275299739134723No Hit
GCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGACCGGCCC59800.12442963680903318No Hit
GCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCG59530.1238678307565509No Hit
CGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCC59120.12301471786204082No Hit
CGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGG58920.12259856523057248No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCACCCCCACGC57990.1206634554942447No Hit
GGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGACCGG57970.12062184023109788No Hit
GGCGGAGGCGAGCCCTGGATAGGCCACCCGTCGGAGCCCGGCGACACCCG57280.11918611365253212No Hit
GGGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGGGGGGA56840.11827057786330177No Hit
GGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGCG55960.1164395062848411No Hit
GCGGCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCGAGCACA55800.1161065841796664No Hit
GGGAACCCACGACCGGCACCGACAGGACCGACCCGCGGAAATCGGGCAAG55090.11462924233795382No Hit
CGCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTG54770.11396339812760448No Hit
CCGGCCGCACCCCGTTTCCCAAGACGAACGGCTCTCCGCACCGGACCCCG53600.11152890523351469No Hit
GCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGACCAGGCTCACGCG53070.11042610076012359No Hit
GGAGACGAAGAAGAGCCACGGGGGCCACGGGCCCCGACCGCCCCTAGCGG51990.10817887655019456No Hit
GGCGACGACGGCAAGGGCGGCCGAAACGGGCCCCTCCCGGAAGGGGGTCC51970.10813726128704772No Hit
TGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA50520.10512015470890228No Hit
CCGGGTGAAGGAGAAGCGGAGACGAAGAAGAGCCACGGGGGCCACGGGCC50220.10449592576169976No Hit
GGGAGCGGAAAAACACGGGAACGACACGGGAACCCACGACCGGCACCGAC49950.10393411970921751No Hit
CCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCT49740.10349715944617575No Hit
CCGCCTCCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGG49680.10337231365673526No Hit
CGCACCCCGTTTCCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCC49170.10231112444649099No Hit
GGCGCCATTGCGCGAGAGATGGAAGGACGGAGGAGGGGAGGTCGACGAGC49110.10218627865705047No Hit
GGACGCGGGACGCCAAACCCTCCCTCGCAACGCCACCGGGGATACCACCT48960.10187416418344923No Hit
GCCAAACCCTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCT48770.10147881918355431No Hit
GGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGCACA48480.10087539786792522No Hit
GCGCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCT48350.1006048986574708No Hit
GCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGG48190.10027197655229614No Hit
CTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCG48080.10004309260498855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT49400.041.5509744
GATCGGA78950.041.447231
TATGCCG50100.041.40954643
ATCGGAA85700.038.4566152
TCGGAAG86450.038.1476363
GTATGCC56200.037.11064542
TACACGA27050.031.63798342
AGTCTAC29450.030.92597825
TACACTC27950.030.38182629
CAGTCTA30100.030.18505324
CTACACT29300.029.58264528
GTCTACA31450.028.95930326
CACTCTT28650.028.56450731
ACTCTTT29000.028.523232
ACACTCT29450.028.08736230
TCTACAC32250.027.7634327
CTACACG31050.027.49138541
CCAGTCT33300.027.35045223
ATTACCG655.9390914E-727.0760320
GGTTCGG28050.025.9610144
Produced by FastQC (version 0.11.2)