FastQCFastQC Report
Mon 3 Dec 2018
G166_M5.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M5.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30653475
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27072278.831713207067061No Hit
GGCGACCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGAC709350.2314093263488071No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTGGAGTTCAGACGTGTG614400.200434045405945Illumina Single End PCR Primer 1 (96% over 32bp)
GAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGGCAGCGCC533270.17396722557556688No Hit
CGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGG504310.1645196833311721No Hit
GGGAACCCACCGCGCCCCCGTGGCGCCCGGGGGTGGGCGCGTCCGCATCT495640.16169129274902763No Hit
TCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC452520.1476243721144177No Hit
GACGATCAATCGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTT443870.14480250607802214No Hit
GGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGC404380.131919790496836No Hit
CGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGGCTCG398340.12994937768067077No Hit
CGGGGGGGGCGGCGCGTCTCAGGGCGCGCCGAACCACCTCACCCCGAGTG395780.12911423582481268No Hit
GGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCAGGG358170.11684482754402233No Hit
CGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGG336840.10988639950282961No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGA321580.10490817109642543No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGG307650.10036382498232255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA165000.038.2288251
ATCGGAA174950.036.381122
TCGGAAG190700.034.1954543
TAGACTG130150.029.02242329
GTAGACT144000.026.82684328
CGAGTGA44250.024.2631633
ATCGACA39750.023.4113835
GACTGGA163550.022.78641931
AGTGTAG234650.022.59456825
ATCATCG41600.022.5827122
TCATCGA42300.022.3129923
AACGCCT115050.021.6280572
TCGACGT55850.021.58771737
CATCGAC46950.021.2740294
TGGAGTT186050.021.04936434
GAGTGTA256200.021.02893324
AGACTGG182150.020.78557830
TGTAGAC221100.020.78534527
TCGTGTA269200.020.70095311
GGAGTTC228600.020.56728235
Produced by FastQC (version 0.11.2)