FastQCFastQC Report
Mon 3 Dec 2018
G166-M13.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166-M13.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50000000
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4317830.863566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA100350.025.54086711
CGTCGTG103400.025.3619589
CGTGTAG101500.025.35986112
AGAGCGT116400.022.9638275
GCGTCGT114300.022.7316388
AAGAGCG119800.022.4774884
AGCGTCG125250.021.1829387
CGGAAGA136800.020.1031461
GATCGGA41700.019.8377271
GAGCGTC137050.019.6482096
GTCGTGT135500.019.28875510
TCGACGT36200.018.96206937
ATCGCGT68350.017.8641179
ACGATCA120400.017.8160972
CACGATC55350.016.8534321
CGATCAC54950.016.6955973
AATCGCG75050.016.4452028
GTAGACT131700.016.16974628
GAGTGTA168400.015.65050524
GACGATC79700.015.6242151
Produced by FastQC (version 0.11.2)