FastQCFastQC Report
Mon 2 Oct 2017
WT_WY_3_S11_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_WY_3_S11_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23102892
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC61350.028.0091843
GCGTCGG85550.020.085855145
CACGATC99550.018.9903031
TAACGCG18600.016.3742127
GTTTCGG57700.014.7020124
CCACTAG89650.014.557573
ACGATCA119600.014.549522
CGTTGAT30700.014.0277191
CGTTAAT13850.013.1753941
TCTAACG22050.013.1535085
TCGTTGC113350.012.6666048
GATATAC88700.011.9349185
CTCGTTG119250.011.9185047
ATATACC90850.011.8922946
CACTAGC109000.011.8402324
AATCGTG199200.011.8299875
TCTCGGG132700.011.747069145
GTTGATT90150.011.5782711
TTGATTA78700.011.423932
CGTGGAT87750.011.3958531
Produced by FastQC (version 0.11.2)