FastQCFastQC Report
Mon 2 Oct 2017
WT_WY_1_S13_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_WY_1_S13_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21628895
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT220530.10196082601538359No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGAA289650.029.660618145
CGAATGT90800.023.4717063
CTCGAAT127650.022.9118671
ACGTTTA24400.019.0139944
CTCCTAT91750.018.8723561
CGCCTAT9350.017.896641
TCGGTTT87700.017.277232
CTCCGAT135350.016.7169631
CGTTTAC56800.016.208195
TCCGATT95950.016.1694832
TCGAATG132500.016.1411132
CGGTTTG94300.016.066373
CGTATAT32100.015.8079723
CCCGTTT48800.015.8030651
CTCCGTT81650.015.7714761
GTCGGTT105350.015.6764091
CGGGTTA34150.014.8605442
GTCCGTT40550.014.53280451
CCGATTA91950.014.5060543
GTTCGAT64400.014.4603681
Produced by FastQC (version 0.11.2)