FastQCFastQC Report
Mon 2 Oct 2017
WT_CON_2_S15_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_CON_2_S15_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22873700
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT311590.13622194922552974No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT304860.13327970551331877No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG252390.1103406969576413No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC242470.10600383846950864No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC230080.10058713719249619No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT123650.021.2356221
CGAATGT112350.020.7116493
GGGTTCG53850.018.307793
TCGGTTT91300.017.4698312
TCGATTC59950.017.4133227
CGCCATT39550.017.1036341
TTCGATT81550.017.0692352
TCCCGAA232300.016.353271145
GTCCGGT35500.015.9815241
AGCTTTA264450.015.734434
ACTAATC45750.015.6884822
GTTCGAT94250.015.66630651
TATCATG88350.015.5899926
TCGATAA21950.015.5219413
GGGTAAT154500.015.1593071
CGGTTTG95600.014.86212353
GTCGTTT87900.014.8120821
CCGATTA80850.014.6147123
CTCCTAT79700.014.5107791
GTCCGTT38600.014.3211611
Produced by FastQC (version 0.11.2)