FastQCFastQC Report
Mon 2 Oct 2017
G23Rik_WY_2_S6_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG23Rik_WY_2_S6_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22426989
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG344100.15343120737250998Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACGA84050.030.7459811
TCTAACG40000.030.6352185
CGATCAC104700.029.5703263
CCACGAT110900.023.406062
CACGATC148450.022.8171081
AACGCGT62100.020.9016178
ACGATCA151650.020.750292
CTAACGC59550.020.5786386
TAACGCG60350.020.5471637
ACGCGTG62250.020.1527779
CCACTAG80700.016.4419173
TCACAAG215400.015.0141556
AGTCTAA86800.014.2005163
CACTAGC96050.014.1162434
ACTAGCC113850.012.5466415
GATCACA268750.012.2754624
GTTGATT83700.012.1230571
GAGTCTA114900.011.6111342
CGTGGAT86150.011.5240841
GTTTCGG56200.011.4822924
Produced by FastQC (version 0.11.2)