FastQCFastQC Report
Mon 2 Oct 2017
CBF_WY_2_S1_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBF_WY_2_S1_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24195062
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG44500.033.077215
CGATCAC73800.024.2668133
TAACGCG64350.023.2136237
CTAACGC67000.022.4028666
AACGCGT68100.021.9346568
ACGCGTG68850.021.274759
GCCACGA76150.019.4592911
CCACTAG88600.017.5126633
CACGATC127200.016.2979161
AGTCTAA97250.015.3585343
CACTAGC106400.014.7873474
CCACGAT98700.014.5452462
GCGTCGG162750.013.988522145
ACGATCA133500.013.5778742
GAGTCTA121600.012.9389542
GTTGTGG202000.012.3949171
ACTAGCC131650.012.3372955
TCGTTGC130950.012.293018
CTCGTTG136100.012.2544537
TCGCCGG65100.012.028346145
Produced by FastQC (version 0.11.2)