FastQCFastQC Report
Mon 2 Oct 2017
CBF_WY_2_S1_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBF_WY_2_S1_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24195062
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTA29300.024.2455644
TGTCTAA146400.020.8462245
GGGTTCG64050.019.5794663
TAAATTC170900.018.6230229
CGTATCG24950.017.4322933
CTAAATT186650.016.779228
GTCCGGT36750.016.031731
TATCATG89000.015.8836996
TCGGTTT100800.015.679582
TCTAAAT204700.015.6184087
ACGTTCG21900.014.5651716
GTCGTTT104900.014.4918391
GTCGGTT131750.014.46449851
GTCCGTT44450.014.2364021
TCGATTC91550.014.1745977
TTGTCTA230550.013.928764
GTCGGAT46850.013.8176161
GTCTAAA220550.013.7725386
TTACCGG49250.013.6896628
ATCATGC110350.013.53352457
Produced by FastQC (version 0.11.2)