FastQCFastQC Report
Mon 2 Oct 2017
CBF_WY_1_S2_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBF_WY_1_S2_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24764876
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG43900.031.382415
CTAACGC60950.023.0801836
TAACGCG65300.022.2098487
AACGCGT65450.021.4936588
CGATCAC66700.020.9799333
ACGCGTG66900.020.8110649
CCACTAG93350.016.5439013
GCCACGA71300.016.0763021
GCGTCGG167050.015.451759145
CACGATC122550.014.3575311
AGTCTAA97700.014.1746223
CACTAGC112200.014.1522564
CCACGAT95800.012.269771
ACGATCA129300.011.7198292
ACTAGCC139550.011.4831135
GAGTCTA127250.011.2818652
GTCTAAC126450.011.1239064
AATCGTG189150.010.848695
CGTGGAT97050.010.6824011
TCTCGGG160350.010.671303145
Produced by FastQC (version 0.11.2)