FastQCFastQC Report
Mon 2 Oct 2017
CBF_WY_1_S2_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBF_WY_1_S2_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24764876
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTAA155150.021.8199445
TAAATTC178300.019.3138339
ACGTTTA25050.019.0994664
CTAAATT183200.018.4405738
TCTAAAT200150.017.3497777
TCGGTTT99250.016.8742122
GGGTTCG58950.016.7239993
GTCTAAA205300.016.4551966
TTACGGA60100.015.4400778
TTGTCTA226950.015.3638454
GTTACGG59600.014.8397837
GTCGGTT125100.014.8292811
CGTATCG28450.014.7785133
GTCCGGT36150.014.6910131
TTACCGG53200.014.5809598
GTCCGTT45600.014.358691
AGTTACG83950.013.9030896
CGGTTTG109400.013.7825973
GTCCTAT68400.013.6141671
CCGTATC75300.013.3832592
Produced by FastQC (version 0.11.2)