FastQCFastQC Report
Mon 2 Oct 2017
CBF_CON_2_S3_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBF_CON_2_S3_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22495125
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT295490.13135734964797927No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT232740.10346241685698569Illumina Single End PCR Primer 1 (100% over 50bp)
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT231830.10305788476392107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACGA80150.036.0687071
CGATCAC133150.035.2865753
TCTAACG38600.031.182645
CCACTAG102850.030.595753
CCACGAT117700.030.0818211
CACGATC173350.029.4790211
ACGATCA162450.029.1899742
CTAACGC54150.023.2999736
CACTAGC136150.023.16584
AACGCGT53700.022.8210898
ACGCGTG57350.021.8745219
TCACAAG246900.019.8825576
TAGCCTC169200.018.9002697
TAACGCG67550.018.8937027
ACTAGCC178800.017.5595135
GATCACA289650.017.047054
CCCACTA189100.016.679132
CTAGCCT208800.015.1064856
CCCCACT235350.014.8890131
AGTCTAA90150.013.5924163
Produced by FastQC (version 0.11.2)