FastQCFastQC Report
Mon 2 Oct 2017
CBF_CON_1_S4_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBF_CON_1_S4_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23305771
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT339520.14568065566249663No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT311910.13383380451133756No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT291480.12506773536906374No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC282410.12117599542190644No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA251200.10778446248356253No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGT236320.10139977776319865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACGA89600.048.7280851
CCACGAT129750.033.6407932
CGATCAC158450.032.9017073
TCTAACG39600.032.5934875
ACGATCA182400.029.1377662
CACGATC190450.028.6752221
CCACTAG95800.024.8250483
AACGCGT57150.023.092978
CTAACGC58050.022.7346956
ACGCGTG60600.022.1377759
TAACGCG62550.021.3319287
TCACAAG262100.020.2518226
CACTAGC125400.019.023134
GATCACA321750.016.9015434
GCGTCGG156750.015.726485145
CGTTGAT34900.015.4807661
AGTCTAA89150.015.0463843
TAGCCTC168200.014.8741687
ACTAGCC176650.013.7100435
GAGTCTA103550.013.0238672
Produced by FastQC (version 0.11.2)