FastQCFastQC Report
Mon 11 Sep 2017
G160-M6-Index6_GCCAAT-_ACB0DPANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M6-Index6_GCCAAT-_ACB0DPANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044330
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGTACGTCGGGAAAAAAAAAAGAGCGAGAGCGCCAGCTATCCTGAGG66820.21949000272637986No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA63690.20920859433767036No Hit
CTGCGTACGTCGGGAAAAAAAAAAAGAGCGAGAGCGCCAGCTATCCTGAG62810.20631797472678717No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT57290.18818590625851994No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT40300.1323772389984003No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT39120.12850118088380694No Hit
CACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTACTAAA39070.12833694113318858No Hit
GCCGGATAAAACTGCGTACGTCGGGGAAAAAAAGAGCGAGAGCGCCAGCT38460.12633321617564455No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT38260.1256762571731711No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA34500.11332542792667025No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA33200.10905519441059282No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA31660.10399661009154725No Hit
CCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATA30930.10159870973251914No Hit
CTAACAATGAATTTTCACATATAAGTTGGATTTTAATTCTATTTATTTAT30910.10153301383227178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGAT11550.033.176791
GATCGGA4600.024.8950231
TATCTCG2350.024.35201539
CGCCAAT2800.021.21102533
CCGGATA26550.020.6439632
GCTGCGT5550.019.840111
CGTGTAG2600.019.46596515
ACGCCAA3350.019.03869632
CTCGCTA5600.018.8764481
ATCTCGT3050.018.76396240
TCACGCC3200.018.5473630
AGTCACG3450.018.47491628
ATCGGAA6200.018.4616242
TACGTAT5650.018.3108142
GTACGTA5550.018.2529011
TGCGGCG2800.018.0812322
ACGTATA5850.018.0649683
CTCGTAT3550.017.98575442
CGTCTGA3550.017.97413316
TCTCGTA3350.017.74302141
Produced by FastQC (version 0.11.2)