FastQCFastQC Report
Mon 11 Sep 2017
G160-M6-Index6_GCCAAT-_ACB0DPANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M6-Index6_GCCAAT-_ACB0DPANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044330
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGTACGTCGGGAAAAAAAAAAGAGCGAGAGCGCCAGCTATCCTGAGG65330.21459565815795265No Hit
CTGCGTACGTCGGGAAAAAAAAAAAGAGCGAGAGCGCCAGCTATCCTGAG63220.20766474068185775No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA62790.20625227882653985No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT58360.1917006369217529No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT41200.13533355450953083No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT38840.12758143828034413No Hit
CACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTACTAAA37500.12317981296377199No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT36490.11986217000128108No Hit
GCCGGATAAAACTGCGTACGTCGGGGAAAAAAAGAGCGAGAGCGCCAGCT36350.11940229869954964No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA35320.11601895983681138No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA31790.10442363344315497No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA30890.10146731793202445No Hit
CTAACAATGAATTTTCACATATAAGTTGGATTTTAATTCTATTTATTTAT30660.10071181507917998No Hit
CCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATA30520.10025194377744856No Hit
GGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAAAG30490.10015339992707754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGAT11050.032.0807881
GATCGGA5100.022.018181
TATCTCG1950.021.44756139
CCGGATA23550.020.7470422
TGCGGCG2250.020.541442
CGTGTAG3300.020.00490815
GTACGTA6850.019.607431
CGCGTAC1504.620233E-1019.0824221
CTCGCTA5100.018.9960771
TACGTAT7050.018.7307952
TCGCTAT5200.018.6227322
CCGGTAA3350.018.4090644
GCTAAGC15900.018.00527442
ACGTATA7250.017.915273
TACGTTC3200.017.85599127
ATTTAGG15800.017.80117829
CTAAGCA16050.017.70126243
CGGAAGA6600.017.347374
TATCCGA12650.017.05331638
ATCGGAA6650.016.878842
Produced by FastQC (version 0.11.2)