FastQCFastQC Report
Wed 30 Aug 2017
G160-M55-Index5_ACAGTG-_ACB0DPANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M55-Index5_ACAGTG-_ACB0DPANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25873220
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG763300.2950154638657268No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC553600.21396641005642125No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC549600.21242040998375927No Hit
GTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTT525090.20294729453852284No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG404040.15616146733958897No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA353830.13675530142749917No Hit
CTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCA342830.13250380122767866No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT334570.1293113110776316No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG319090.12332829079642967No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGG312900.12093585568398522No Hit
CTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGC300700.11622055546236612No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC296370.1145470103837095No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC292230.11294690030850432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACGT120200.023.6644133
GCAGACG123850.022.9498562
ACGTTCG129850.021.8888156
GCCGGAT16100.021.8861941
GACGTTC137200.020.8605085
CGTTCGA139000.020.7802937
AGACGTT138300.020.4718974
GTCGGAT61950.019.943331
GTCGGTA10300.019.6710131
TGCGGTA82950.019.572822
TGTTACG61800.019.4715523
TCGGATT64800.019.181692
GGCAGAC164700.019.121351
CGCTCCG41600.018.4038143
GTTACGA66000.018.3324474
TTACGAC66550.018.1148265
GCGACGT45450.017.95736522
GTCGTCG161750.017.95279918
CGTCGCC162650.017.86698520
GGGTCGT164650.017.6900416
Produced by FastQC (version 0.11.2)