FastQCFastQC Report
Wed 30 Aug 2017
G160-M44-Index4_TGACCA-_ACB0DPANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M44-Index4_TGACCA-_ACB0DPANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19646773
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1921470.978007940540668No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA974940.49623416527487746No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC789690.4019438713930273No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC708760.36075135596059466No Hit
GTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTT697520.35503031464760143No Hit
GTCGGGAGGAACGGGGAGCGGGAAAGATCCGCCGGGACCACCGGCACGGC643020.32729039013175343No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT617030.3140617545690582No Hit
CTAACGCGTACGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG570440.29034793652881313No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG569580.2899102056098475No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT563910.28702423548131795No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG553050.2814966101557747No Hit
GCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAG534080.27184108046649696No Hit
CTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCC520710.2650358916449027No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGG517060.26317808018650185No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT504880.25697858879929036No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTA504040.25655103766913784No Hit
CCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAA484090.2463966983280155No Hit
CTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGC472790.24064511764858282No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC471450.2399630717981014No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAG470150.23930138552524632No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT454780.23147821782233655No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCCGGGAAGGGGGGTCAGCG454440.23130516141251287No Hit
CCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC447330.2276862464894362No Hit
GTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGCCCGC445090.2265461101423628No Hit
AGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCA432930.22035679854396445No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA422410.21500222962824478No Hit
CTTCCGTACGCCACATTTCCCACGCCGCGACGCGCGGCGGGGATTCGGCG419370.2134549017286452No Hit
CTACGAATGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGAC411090.20924046915999894No Hit
TGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGC409080.20821740038427686No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT397410.2022774936118008No Hit
CTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCA396680.2019059313201206No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC395950.20153436902844044No Hit
GTCGGATTCCCCTGGTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGG394010.2005469295135644No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG392630.1998445240854567No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT383370.1951312818649658No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC373870.19029588217871707No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG371670.18917610540927002No Hit
TGGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGG363770.1851550888280737No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC356660.18153617390499702No Hit
CTGCGTACGTCGGGGAAAAAAAGAGCGAGAGCGCCAGCTATCCTGAGGGA350790.17854840588833598No Hit
CTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGG346490.1763597512935076No Hit
GGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGC345030.17561662671014727No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGG341600.17387079292869115No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC340500.17331090454396758No Hit
AGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGA338270.1721758580913008No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA335810.1709237440672827No Hit
GCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGCGTG327240.1665617045608457No Hit
GGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGT325500.16567606293410117No Hit
CTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAAC321760.16377244242604116No Hit
GAAAAAAAGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAA319350.16254577787405594No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG319300.16252032840202307No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA316870.16128348406122472No Hit
CGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATA313650.15964453806230672No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC313040.1593340545035055No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC307960.15674838814496406No Hit
ACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG307320.1564226349029431No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG306980.15624957849311946No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGA305230.1553588469719684No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCCGGGAAGGGGGGTC304100.1547836889040251No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT302500.1539693057989727No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT298540.15195370761396795No Hit
CCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGT295240.15027404245979734No Hit
GGGAACGACGCGCGGGTGGAGGGGTCGGGAGGAACGGGGAGCGGGAAAGA293680.14948001893237126No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC292600.14893031033646084No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC292020.14863509646087936No Hit
CTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAA290150.14768328620684934No Hit
GCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAA288380.1467823748968851No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA287900.14653805996536937No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT287730.14645153176045755No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTACGCTCGTGCTC286210.14567786781065775No Hit
CTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGA284780.14495001291051718No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG284430.14477186660628696No Hit
CCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCCGGGAAGGGG283140.14411527022783843No Hit
GGAAAAAAAGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGA278540.14177391880081275No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCG275070.1400077254417303No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC274610.13977359029902775No Hit
CGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGG273180.13904573539888712No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC270530.13769691338114406No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC269930.13739151971674943No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG269670.1372591824621784No Hit
CGAATGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGG269550.13719810372929947No Hit
CCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCC264010.13437830222805547No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC260100.13238815351508362No Hit
GTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGCATCGG254760.12967014990197118No Hit
GGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTAC252940.12874378911997406No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG250640.1275731134064612No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC248390.12642788716498124No Hit
GTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAA246720.12557787479908278No Hit
CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTC246520.12547607691095122No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA244470.12443264855760282No Hit
CCGAGAGGCAAGGGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCG244410.12440210919116336No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC242540.12345029893713333No Hit
CCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCT241620.1229820286517282No Hit
GTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTT236630.12044217134284597No Hit
CTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGGA236090.1201673170448908No Hit
CCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGA233510.11885412428799375No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA232760.11847238220750043No Hit
TGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGC232440.11830950558648996No Hit
GCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGA226250.11515886094881841No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT226180.11512323168797237No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC226050.11505706306068686No Hit
CGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCAT225120.11458370288087515No Hit
GCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC224940.11449208478155676No Hit
GTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA224100.11406453365140423No Hit
CCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG222750.11337739790651626No Hit
CGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC222340.11316871223584657No Hit
CCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGG221440.11271062173925459No Hit
CTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGAC220150.11205402536080607No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT219860.11190641842301532No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG219760.11185551947894956No Hit
CCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCG218340.11113275447321552No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA216780.11033873094578941No Hit
GGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACG212190.10800246941317031No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT210180.1069794006374482No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT209990.10688269264372321No Hit
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGAC209630.10669945644508642No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCG209100.10642969204153782No Hit
GGCGAGACGGGCCGGTGGTGCGCCCTCGGCGGACTGGAGAGGCCTCGGGA206510.10511140939023422No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG206150.10492817319159742No Hit
GGGAAAAAAAGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGG205370.10453116142788436No Hit
GGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTA205260.10447517258941202No Hit
GCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCA205090.1043886443845002No Hit
GGAGGAACGGGGAGCGGGAAAGATCCGCCGGGACCACCGGCACGGCCGGA205050.10436828480687389No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT204090.10387965494384242No Hit
GGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTT204070.10386947515502928No Hit
GCCGAGAGGCAAGGGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACC202360.1029991032115045No Hit
CCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTG201730.10267843986389012No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC201080.10234759672746256No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC200810.10221016957848497No Hit
GGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTT199990.10179279823714561No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA199360.10147213488953123No Hit
CGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAG198750.10116165133072998No Hit
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGACTGGAGAGGCCTCGGGAT198680.10112602206988394No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC198520.1010445837593787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGAT17000.034.4567341
GTCGGTA7100.034.1173821
ACCAATC12900.032.40389636
CTCGTAT14150.031.7177742
CGTCTGA14850.031.70230516
GACCAAT13650.031.42934435
ACGTCTG15450.030.32875815
GACGGTT5650.030.011221
CGCGGTA3550.029.7751711
CTGACCA15150.029.76970533
CCAATCT14150.029.54135937
CACTGAC15500.029.5229331
TGACCAA15550.029.2868934
GCGACTT9700.029.0589311
GCCTGTT17450.028.8989091
GCGCTAT2000.028.6275861
CAGTCAC16700.028.19065727
GCGGTTT7250.027.9441681
GTTCGCT5600.027.9197641
GAACTCC16850.027.6782321
Produced by FastQC (version 0.11.2)