FastQCFastQC Report
Mon 11 Sep 2017
G160-M4-Index4_TGACCA-_ACB0DPANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M4-Index4_TGACCA-_ACB0DPANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259208
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC47160.20874571973895276TruSeq Adapter, Index 4 (100% over 50bp)
GCCGGATAAAACTGCGTACGTCGGGGAAAAAAAGAGCGAGAGCGCCAGCT43890.19427162085120095No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA42260.1870567030570005No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG38840.17191865467898484Illumina Single End PCR Primer 1 (100% over 50bp)
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT33280.14730826023987167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG6500.034.536539
GATCTCG6750.032.92027734
GAGTGTA7950.032.6186427
ATCTCGT6900.032.5358440
GCCGGAT7800.032.471021
CTCGTAT7100.032.25880442
TCTCGTA7200.031.49854941
GTCGCCG6550.031.27396444
ATCTCGG7350.031.13369635
GTAGATC7650.030.75088531
TAGATCT7650.030.47302432
CGTGTAG8900.030.16228515
GATCGGA16750.029.7157251
ACGTCTG8150.029.69815615
CGTCGTG8950.029.50603912
GCGTCGT9000.029.34211311
TCTCGGT7650.029.33883536
CGGAAGA16950.029.2351634
GGTCGCC7150.028.64765443
CCAATCT7950.028.50976637
Produced by FastQC (version 0.11.2)