FastQCFastQC Report
Mon 11 Sep 2017
G160-M4-Index4_TGACCA-_ACB0DPANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M4-Index4_TGACCA-_ACB0DPANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259208
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC47070.20834735004479446TruSeq Adapter, Index 4 (100% over 50bp)
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA43090.1907305569031271No Hit
GCCGGATAAAACTGCGTACGTCGGGGAAAAAAAGAGCGAGAGCGCCAGCT42050.18612717377063112No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG37980.16811201093480546Illumina Single End PCR Primer 1 (100% over 50bp)
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT33360.14766236663467905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT7550.033.82246840
AATCTCG7650.033.3781139
GCCGGAT7400.032.7378161
GATCTCG5550.032.50883534
ATCTCGG5600.032.22073735
GTCGCCG5300.031.58174944
CTCGTAT8650.030.80554242
TCTCGTA8300.030.77448841
GTAGATC6200.030.49563831
CGTGTAG7150.030.16123615
TAGATCT6350.029.4402332
GACCAAT9000.029.33920935
GATCGGA17000.029.2784611
GAGTGTA7200.028.9966227
TCTCGGT6150.028.6261836
GTGGTCG5800.028.4737241
GGTCGCC5850.028.23414843
TGGTCGC5800.028.09721842
TGTAGAT7050.027.74385830
AGAGCGT7900.027.5739088
Produced by FastQC (version 0.11.2)