FastQCFastQC Report
Wed 30 Aug 2017
G160-M22-Index2_CGATGT-_ACB0DPANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG160-M22-Index2_CGATGT-_ACB0DPANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26215440
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA46450.030.45487214
CGTGTAG47650.029.91876215
CGTCGTG48750.029.24490412
GATCGGA52400.027.2604311
ATCGGAA55250.025.9755882
AGAGCGT56000.025.9683368
ATCTCGG48450.025.65495535
TGGTCGC44800.025.54048542
AGCGTCG60850.025.23740210
AAGAGCG58200.024.9482447
GATCTCG49350.024.60699334
GAGCGTC66500.023.191769
TCGGAAG69950.020.8325653
GCGTCGT73900.019.76848811
CGGAAGA73400.019.6742674
GTAGATC67900.018.65887331
GTGGTCG64900.017.66384341
GTAGGGA82200.017.55488218
TGTAGGG87950.016.68317
GTCGTGT86800.016.62776613
Produced by FastQC (version 0.11.2)