FastQCFastQC Report
Tue 27 Jun 2017
G155-M7-Index25_ACTGAT-_ACB6A7ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M7-Index25_ACTGAT-_ACB6A7ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20644484
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6050.011.273291
CTCGTAT6700.08.86501944
TCTCGTA8301.8189894E-127.421211743
ATCGGAA9400.07.25569872
TATCTCG10053.074092E-106.129169541
ACACGTC13501.8189894E-125.70346313
ACGTCTG13901.8189894E-125.69765615
ATCTCGT12604.4019544E-105.41244442
CGTCTGA12654.8930815E-105.391142416
Produced by FastQC (version 0.11.2)