FastQCFastQC Report
Tue 27 Jun 2017
G155-M6-Index23_GAGTGG-_ACB6A7ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M6-Index23_GAGTGG-_ACB6A7ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21899737
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8450.013.7992181
CTCGTAT8800.012.99907244
TCTCGTA8550.012.35027843
ATCGGAA10150.012.1381182
GTCACGA11450.09.79827129
CGTCTGA12350.08.90693916
ACGAGTG12300.08.585772532
CACGAGT13700.08.51111831
ACACGTC13800.08.44890413
TCACGAG14500.07.888965630
CGAGTGG14850.07.852187633
TATCTCG12950.07.474950341
TCGGAAG17300.07.37583543
ACGTCTG15250.07.21306115
AGTCACG17250.06.88635528
ATCTCGT15400.06.57134942
CGGAAGA20200.05.98974044
CACGTCT23350.05.46451514
GCACACG27650.05.17157411
AATCGAC7352.9467212E-45.088676532
Produced by FastQC (version 0.11.2)