FastQCFastQC Report
Tue 27 Jun 2017
G155-M2-Index19_GTGAAA-_ACB6A7ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M2-Index19_GTGAAA-_ACB6A7ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21249678
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA7250.09.1036081
CTCGTAT8050.07.92497244
ATCGGAA9750.06.99492932
AACGATC7354.0272425E-96.884491438
TCTCGTA8757.094059E-116.788212343
CGTCTGA11250.06.64880316
CGATCTC9006.4574124E-96.11114740
ACACGTC14700.05.83641213
ACGATCT9854.6613422E-85.583815639
GATCTCG10302.1087544E-85.553378641
ACGTCTG13301.8262654E-95.127656515
GTCACGT16903.6379788E-125.076546729
ATCTCGT13106.6447683E-95.038019742
GAAACGA14001.3260433E-95.02889736
TGAAACG13208.030838E-95.00035935
Produced by FastQC (version 0.11.2)