FastQCFastQC Report
Tue 27 Jun 2017
G155-M13-Index5_ACAGTG-_ACB6A7ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M13-Index5_ACAGTG-_ACB6A7ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25090339
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT9150.012.021605542
GATCGGA9450.011.4078511
TCGTATG9900.010.66625143
CGTATGC10750.010.43667844
TCTCGTA9450.010.24327441
ATCGGAA10850.09.7331542
GATCTCG13450.08.17847839
CGTCTGA13850.07.62425216
ACACGTC16000.07.42454813
ATCTCGT15250.06.63604540
TCGGAAG18250.06.50981573
ACGTCTG18200.06.40631815
Produced by FastQC (version 0.11.2)