FastQCFastQC Report
Tue 27 Jun 2017
G155-M1-Index18_GTCCGC-_ACB6A7ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M1-Index18_GTCCGC-_ACB6A7ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21101671
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG4200.019.38219812
AGCGTCG4800.018.33407610
GCGTCGT5200.015.65448211
GATCGGA8350.011.0741431
ATCGGAA9800.010.1095712
GAGCGTC10000.09.4603179
CGTGTAG10800.08.96317715
GTCGTGT8850.08.94957913
TCGTGTA9700.08.84563514
GTCGCCG6707.2759576E-128.20926944
TGGTCGC8251.8189894E-127.46668642
AAGAGCG14650.07.058447
GTGGTCG8503.6379788E-116.98807141
AGAGCGT13150.06.5250268
TCGGAAG16050.06.0356343
GATCTCG11302.3646862E-116.03336834
CGGAAGA19350.05.68885764
TCGGTGG15001.0913936E-115.279162438
ATCTCGG14501.2369128E-105.15692235
TCTCGGT15006.184564E-115.131886536
Produced by FastQC (version 0.11.2)