FastQCFastQC Report
Tue 27 Jun 2017
G155-M1-Index18_GTCCGC-_ACB6A7ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M1-Index18_GTCCGC-_ACB6A7ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21101671
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG5100.020.27527232
CGTCCGC6600.015.00052833
GATCGGA6850.013.8108691
CTCGTAT7850.012.05029544
TCTCGTA7950.011.34542543
GTCCGCA9750.010.83117734
TCCGCAC10300.09.82563535
ATCGGAA10050.09.632182
CGTCTGA13850.07.94219816
CCGCACA13800.07.493035336
CGCACAT13300.07.44388937
ACACGTC13300.07.277934613
ACGTCTG14350.07.2054715
AGTCACG16800.06.939980528
ATCTCGT13800.06.536167642
GTCACGT17500.06.285265429
TCGGAAG16300.06.20874933
CATCTCG15300.06.183048241
CGGAAGA18400.05.619474
CACGTCT22100.05.27584114
Produced by FastQC (version 0.11.2)