FastQCFastQC Report
Wed 28 Jun 2017
G154-M12-Index12_CTTGTA-_ACB6A7ANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M12-Index12_CTTGTA-_ACB6A7ANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20530397
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG3650.020.4927612
GCGTCGT4150.018.55436111
AGCGTCG4850.016.33018310
GTCGCCG3850.013.71556344
GATCGGA5800.013.2860721
CGTGTAG8050.010.6577415
ATCGGAA8500.010.1021772
GAGCGTC7900.010.0254939
TCGTGTA8350.09.48471114
GTCGTGT8400.08.90448413
TGGTCGC5701.70985E-108.49197742
TCGGAAG12000.07.7061473
AGAGCGT10550.07.2992228
GTGGTCG7751.6898412E-96.813255341
GGTCGCC7453.799505E-86.497248643
AAGAGCG11550.06.4769997
GATCTCG8552.0845619E-96.430619234
TCGGTGG9353.5288394E-106.352200538
CGGAAGA14500.06.22506334
ATCTCGG12851.3096724E-105.476745635
Produced by FastQC (version 0.11.2)