FastQCFastQC Report
Wed 24 May 2017
G153-M8-Index-4_TGACCA-_ACAYD3ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M8-Index-4_TGACCA-_ACAYD3ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15817806
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT3450.015.369051
GTCGGTT30100.014.8999361
TCGGTTT29800.014.8368862
CTCGAAT26350.014.756621
CGAATGT21750.014.2601083
ACCGGTA19250.013.82732727
TTACGGC14100.013.41791140
GTTCCGC42200.013.18838527
GCGCATT42150.013.15479444
AGTTCCG41750.013.11973526
ACGTGCC24600.012.96532820
TACGTCA21900.012.95912932
AATCCGA19700.012.95339424
CGGGCTA9950.012.8783361
TTCCGCC43150.012.79606728
TCCGCCC43200.012.78125729
CCGCCCT43350.012.4325630
ATTGCGA21150.012.377254537
TTGCGAT22350.012.30290938
AATTGCG21650.012.29462236
Produced by FastQC (version 0.11.2)