FastQCFastQC Report
Wed 24 May 2017
G153-M6-Index-21_GTTTCG-_ACAYD3ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M6-Index-21_GTTTCG-_ACAYD3ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21897865
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN505270.23073938943362743No Hit
CTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGT238020.10869552808002059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC15600.015.231883
CCACTAG59750.013.2561963
CACGATC24050.011.70817851
CACTAGC74500.010.5745524
CGCAATT23750.010.5593141
TAGCCTC110150.010.4658662
CCCACTA76450.010.3310712
ACGATCA23400.09.9659432
ACTAGCC82900.09.820525
CTAGCCT122500.09.5716231
CCCCACT100050.09.4106831
TACCGGT23850.09.40782610
ACGCTAA10700.09.251826
CGAAAAT35150.09.1373861
CGATACA31850.09.11040736
TCTTCGT120650.09.04909944
TAGATCC31750.09.0085313
TACGCAA13700.08.9918899
TTAGATC31850.08.979712
CTTAGAT39950.08.9205721
Produced by FastQC (version 0.11.2)